DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - hypertension: Heart Ventricle, right, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Epoch Life Science Inc GenCatch™ Plant Genomic DNA Purification System (50 preps) Uniquely formulated buffers, shearing tubes, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to extract gDNA ready for downstream use from various plant species. Comes with shearing tubes for simple and fast homogenization and filtration of plant tissues. 40 ug yield. 100 mg plant tissue. 200 ul elution volume. Designed predominantly for gDNA of 20-30 kb. No phenol/chloroform extraction nor ethanol precipitation required. Used by labs around the world. 18 month shelf life.
New England Biolabs, Inc. NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® – 24 reactions
The NEBNext Ultra II FS DNA Library Prep Kit for Illumina provides a fragmentation system: a fast and reliable solution for DNA fragmentation and library construction. A new DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 pg–500 ng), or GC content. The kit also includes the NEBNext Ultra II Ligation Master Mix for adaptor ligation, and the NEBNext Ultra II Q5 Master Mix for uniform, high-fidelity library amplification.
- Perform fragmentation, end repair and dA-tailing with a single enzyme mix
- Experience reliable fragmentation with a single protocol, regardless of input amount or GC content
- Prepare high quality libraries from a wide range of input amounts: 100 pg-500 ng
- Use with DNA in standard buffers (TE, Tris-HCl) and water
Zymo Research Corporation Quick-DNA/RNA FFPE Kit (50 Preps)
The unique chemistries of the product have been optimized for maximum recovery of DNA and of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin™ Column technology. The result is high-quality DNA & total RNA (including small RNAs) that is DNA-free and is ready for RT/qPCR, hybridization, sequencing, etc.
Research Products International Corp DNA Clean & Concentrator-5 w/Zymo-Spin I Columns, Uncapped, 200 preps
The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.
Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash residue carryover.
Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
New England Biolabs, Inc. Monarch® DNA Capture Beads - 200 beads
These specialized glass beads are optimized for capturing HMW DNA in the Monarch HMW DNA workflows.
- A component of the Monarch HMW DNA Extraction Kits
- 4 mm borosilicate glass beads
- Optimized for extraction of HMW DNA
- Smooth surface to maximize elution efficiency
- Easy to handle when coupled with the Monarch Bead Retainers
New England Biolabs, Inc. Monarch® gDNA Nuclei Prep & Lysis Buffer Pack - 1 pack
The Monarch gDNA Nuclei Prep & Lysis Buffer Pack contains two lysis buffers used in the Monarch HMW DNA Extraction Kit for Cells & Blood. The Nuclei Prep Buffer is a mild lysis buffer used first to lyse the cell membrane and release the contents of the cytoplasm while the nuclei are kept intact. It is used pre-mixed with the RNase A enzyme included in the kit. After the RNA from the cytoplasm is digested by RNase A, the Nuclei Lysis Buffer, pre-mixed with the included Proteinase K, is used to lyse the nuclei and release the HMW genomic DNA. This 2-step lysis ensures that RNA in the cytoplasm is effectively degraded before the viscous genomic DNA from the nuclei is released. 20 ml of each buffer is included in this buffer pack. The Nuclei Prep Buffer is used chilled to 4°C, and it can be stored at 4°C for convenience if desired.
- Supplied as components of the Monarch HMW DNA Extraction Kit for Cells & Blood
Zymo Research Corporation Quick-DNA™ 96 Kit™ (4 x 96 Preps)
This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, buccal cells, cells from culture, and many biological liquid samples.For processing, simply add the specially formulated Genomic Lysis Buffer to the samples, vortex, and transfer the mixtures to the wells of the supplied SiliconA™ Plate. There is no need for organic denaturants or Proteinase K digestion because of the unique chemistries featured in the kit. Instead, the product yields high-quality, purified DNA in just minutes. PCR inhibitors are effectively removed during the purification process. DNA purified using the Quick-DNA™ 96 Kit is suitable for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
Zymo Research Corporation ZymoBIOMICS 96 MagBead DNA Kit – Dx (No Lysis Matrix) (2 x 96 preps)
The ZymoBIOMICS DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Qiagen, Inc. DNEASY PLANT MINI KIT (250)
Kit, Plant Mini; DNeasy; For isolation of total cellular DNA from plant cells and tissues, or fungi; DNeasy Plant Mini Kit (250) Contents: 250 DNeasy Mini Spin Columns, 250 QIAshredder Spin Columns. Mini Kits also include: RNase
New England Biolabs, Inc. NEBNext Ultra II DNA PCR-free Library Prep Kit for Illumina - 96 rxn
The NEBNext Ultra II DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of pre-fragmented DNA input amounts into libraries for next-generation sequencing on the Illumina platform all without an amplification step. The fast user-friendly workflow requires minimal hands-on time and maintains excellent library yields and GC coverage without requiring PCR. Dont be biased The NEBNext Ultra II DNA PCR-free Library Prep Kit for Illumina can save you time and minimize amplification-induced sequence bias and errors with a complete solution for PCR-free NGS library preparation. Built on the robust and reliable Ultra II reagents and workflow from New England Biolabs you can trust that your samples are in the best hands your own.
Enzo Life Sciences Human Genomic DNA, Female (100µg)
Human Genomic DNA, Female, is comprised of a pool of ≥ 8 normal donors. It is intact DNA extracted from freshly harvested whole blood of healthy females. DNA is treated with DNase free-RNase to remove residual contaminant RNA. Human genomic DNA can be used for a variety of molecular applications including PCR, southern blotting, and DNA library construction. It can also be used as normal reference DNA for comparative genomic hybridizations (CGH). Alternative name: gDNA. Concentration: 150 - 250 µg/mL in 10 mM Tris-HCl, pH 8.0, containing 1 mM EDTA. Formulation: Liquid. Long Term Storage: -20°C.
Zymo Research Corporation ZymoBIOMICS-96 DNA Kit (lysis Matrix Not Included) (2 x 96 preps)
The ZymoBIOMICS™ innovative lysis system eliminates bias associated with unequal lysis efficiencies1 of different organisms (e.g. Gram-negative/positive bacteria including endospores2 , fungi, protozoans, algae, etc.) making it ideal for microbial community profiling. Unbiased mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads™ and validated using the ZymoBIOMICS™ Microbial Community Standard.In addition, the ZymoBIOMICS™ 96 DNA Kit is equipped with Zymo Researchs proprietary OneStep™ PCR InhibitorRemoval technology, enabling PCR from the most PCR prohibitive environmental samples rich in humic and fulvic acids, tannins, melanin, and other polyphenolic compounds. Coupling state-of-the-art lysis technology with Zymo-Spin™ Technology results in superior yields of ultra-pure DNA ideal for all downstreamapplications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing.
Zymo Research Corporation ZymoPURE Plasmid MiniPrep Kit (400 Preps)
ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 g/l).In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Thomas Scientific DirectAmpT Genomic DNA Amplification Kits, Preparation Solution for DirectAmpT Tissue DNA Extraction & Amplification Kit, 100 reactions
Extract genomic DNA in 15 minutes Tissue, plant or seed Quick isolation and amplification for genotyping Highly specific PCR amplification Eliminates complicated purifications, centrifugation and extractions Denville DirectAmp™ Genomic DNA Amplification Kits contain all reagents for rapid extraction and amplification of genomic DNA from a variety of sources. These kits provide a simple and quick extraction method that eliminates protocol steps that call for liquid nitrogen freezing, enzyme digestions, hazardous organic extractions, column purification, centrifugation or precipitation. Each kit includes Denville Hot Start Blue Mastermix, noted for its highly specific amplification of targeted genomic DNA, without adding loading buffer or tracking dye for gel analysis. Although included as a standard kit component, Hot Start Blue Mastermix is also available separately.